KMID : 1025520210630040919
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Journal of Animal Science and Technology 2021 Volume.63 No. 4 p.919 ~ p.933
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Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells
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Zhang Junfang
Li Qiang Nogoy Kim Margarette Corpuz Sun Jianfu Sun Bin Wang Ying Tang Lin Yu Jia Jin Xin Li Xiangzi Choi Seong-Ho
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Abstract
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We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fetal bovine serum. When the cells reached 80%?90% confluence, we used the differentiation medium with 5% horse serum for differentiation for 96 h. The differentiation medium contained 50 ¥ìM, 100 ¥ìM and 200 ¥ìM POA. Control BSC were cultured only in differentiation media. Compared with the control BSC, the POA BSC significantly up-regulated the expression of paired box 3 (Pax3) and paired box 7 (Pax7) and down-regulated myogenin gene expression (p < 0.01), which indicates a depression in muscle fiber development. However, all POA treatments up-regulated the expression of the adipocyte transcription factors peroxisome proliferator-activated receptor ¥ã (PPAR¥ã), CCAAT/enhancer-binding protein alpha and beta (C/EBP ¥á and C/EBP ¥â), and other genes (p < 0.01) and increased the expression of PAT-family proteins and the concentration of adiponectin in the media. These results indicate that POA can convert part of BSCs into adipocytes.
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KEYWORD
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Adipocytes, Palmitoleic acid, Satellite cells, Bovine, Muscle, Marbling
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